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KMID : 0376219860230010001
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Chonnam Medical Journal
1986 Volume.23 No. 1 p.1 ~ p.8
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Effect of salts on. destroying of the activity of heated antiserum by preserving at 37°C
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Abstract
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The author investigated the effect of salts on destroying of the activity of heated antiserum by preserving at 37°C. For this experiment, the Klebsiella phage-host cell system was used. The following results were obtained.
On preserving, at 37°C, the antiserum heated at 78ºC for 15 minutes, the serum activity was considerably protected by various salts.
When salts protect the activity of heated antiserum, the salts possessing same cations with different anions showed similar protective effects. However, some salts possessing same anions with different actions showed significantly different activities.
When the activity of heated antiserum is destroyed by 37ºC-preserving, the serum activity was continuously destroyed, regardless of presence or absence of salt, until certain times and, thereafter, the destroying was not progressed.
The heated antiserum-protecting activity of NH4Cl was weaker than that of NaCl.
The activity of antiserum diluted in distilled water was reduced to about half level by heating at 78ºC for 15 minutes.
When the activity of heated antiserum is destroyed by 37ºC-preserving, the serum protecting mechanism of salt was discussed and suggested more important role of the cation than anion.
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